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Fig. 2 | BMC Materials

Fig. 2

From: Chitin synthase localization in the diatom Thalassiosira pseudonana

Fig. 2

Images obtained by confocal immunofluorescence microscopy of T. pseudonana. Samples were prepared by immunlocalization of chs7305 in wild type cells using (a) anti-chs7305. b IgG fraction isolated from preimmune serum, and c anti-chs-7305 preincubated with 100-times excess of peptide. Each micrograph is an overlay of two images of the recorded green channel and the red channel (settings according to Fig. 1). The green color represents CFL488 fluorescence (secondary antibody), the red color is caused by chloroplast autofluorescence. The scale bar is valid for images b and c

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